Resonance Raman studies of heme structural differences in subunits of deoxy hemoglobin
β Scribed by Edyta Podstawka; Cynthia Rajani; James R. Kincaid; Leonard M. Proniewicz
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 2000
- Tongue
- English
- Weight
- 157 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0006-3525
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π SIMILAR VOLUMES
Visible resonance Raman spectra in the low-frequency region (200-500 cm-') are reported for hemoglobin ( H b ) reconstituted with heme that is deuterated at the meso carbon atoms (meso-d,). Spectra were obtained in the deoxy form and in the immediate photoproduct of the carbonmonoxide adduct, HbCO.
## Abstract We describe a pumpβprobe Raman spectrometer based on a femtosecond Ti:sapphire laser, an optical parametric generator and two optical parametric amplifiers for timeβresolved studies, with emphasis on the structural dynamics in heme proteins. The system provides a 100βfs pump pulse tunab