Resolving overlap in two-dimensional NMR spectra: nuclear Overhauser effects in a polysaccharide

✍ Scribed by Eriks Kupče; Toshiaki Nishida; Göran Widmalm; Ray Freeman

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 110 KB
• Volume: 43
• Category: Article
• ISSN: 0749-1581
• DOI: 10.1002/mrc.1647

✦ Synopsis

Overlapping resonances in the two-dimensional nuclear Overhauser (NOESY) spectrum of the O-antigenic polysaccharide from Escherichia coli O147 have been resolved by recording a tilted projection of a threedimensional NOESY-HSQC spectrum, where the carbon-13 and proton evolutions are linked together. Through the introduction of small contributions from the appropriate carbon-13 shifts, a cluster of five unresolved proton peaks is separated into its components without the need to perform the full threedimensional measurement, a time saving of at least an order of magnitude.