Resolution of the optical isomers of dansyl amino acids by reversed phase liquid chromatography with optically active metal chelate additives
β Scribed by LePage, James N.; Lindner, Wolfgang.; Davies, Geoffrey.; Seitz, David E.; Karger, Barry L.
- Book ID
- 127057452
- Publisher
- American Chemical Society
- Year
- 1979
- Tongue
- English
- Weight
- 372 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0003-2700
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## Abstract Enantiomeric resolution of dansyl amino acids by microcolumn liquid chromatography with Ξ³βcyclodextrin as a mobile phase additive has been investigated. The separation factor for the enantiomers increased with increasing Ξ³βcyclodextrin concentration as well as with decreasing acetonitri
A method for determining the optical purity of amino acids using HPLC and precolumn derivatization is described. (+)-1-(1-Naphthyl)ethyl isocyanate reacts with racemic amino acids, in high yield, to form naphthylethyl carbamoyl derivatives. The resulting diastereoisomeric pairs were separated on rev