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Resolution of the conformational distribution and dynamics of a flexible molecule using frequency-domain fluorometry

✍ Scribed by Joseph R. Lakowicz; Józef Kuśba; Wieslaw Wiczk; Ignacy GryczynSki; Henryk Szmacinski; Michael L. Johnson


Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
426 KB
Volume
39
Category
Article
ISSN
0301-4622

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Resolution of a distribution of distance
✍ Joseph R. Lakowicz; Michael L. Johnson; Wieslaw Wiczk; Aneela Bhat; Roben F. Ste 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 603 KB

We used frequency-domain fluorescence spectroscopy to examine the intensity decays of a fluorescent donor attached to an acceptor via a flexible alkyl chain. The intensity decay of the indole donor becomes markedly heterogeneous due to energy transfer to the dansyl acceptor. The measured dispersion