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Resolution of a distribution of distances by fluorescence energy transfer and frequency-domain fluorometry

โœ Scribed by Joseph R. Lakowicz; Michael L. Johnson; Wieslaw Wiczk; Aneela Bhat; Roben F. Steiner

Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
603 KB
Volume
138
Category
Article
ISSN
0009-2614

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โœฆ Synopsis

We used frequency-domain fluorescence spectroscopy to examine the intensity decays of a fluorescent donor attached to an acceptor via a flexible alkyl chain. The intensity decay of the indole donor becomes markedly heterogeneous due to energy transfer to the dansyl acceptor. The measured dispersion of fluorescence decay times was used to recover the donor-to-acceptor distance distribution. The distance distribution was found to be characteristic of the molecule, and not the model used for data analysis. The ability to recover distance distributions in solution should be valuable in studies of biological macromolecules.

๐Ÿ“œ SIMILAR VOLUMES

Distance distributions in native and ran
Distance distributions in native and random-coil troponin I from frequency-domain measurements of fluorescence energy transfer
โœ Joseph R. Lakowicz; Ignacy Gryczynski; Herbert C. Cheung; Chien-Kao Wang; Michae ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 523 KB

We used time-dependent fluorescence energy transfer to determine the distribution of donorto-acceptor distances in native and denatured troponin I(Tn1). The single tryptophan residue (Trp 158) of TnI served as the donor (D), and the acceptor (A) was a labeled cysteine residue (Cys 133). The time-dep