Counterflow centrifugal elutriation (CCE) has been used to fractionate cell populations on the basis of sedimentation properties, with minimal perturbation of metabolic function. Therefore, it is an ideal method for the isolation of cell cycle phase specific populations. We present modifications of
Resolution of cells by centrifugal elutriation
β Scribed by Wilson D. Grant; Martin Morrison
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 270 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0003-2697
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An initial preparation of rat hepatocytes containing approximately 30% couplets was enriched by centrifugal elutriation. Of the couplets loaded onto the elutriator, 87% were eluted at medium flow rates of 60 to 80 ml/min at a rotor speed of 1.100 rpm; cells eluted in this range maintained a viabilit
Minor alterations of the electrical circuitry of the centrifuge required for centrifugal elutriation are described. Furthermore it is shown that elutriation can be performed by hydrostatic pressure without the utilization of a pump. This approach enables us to fractionate human blood cells at a cons
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