✦ LIBER ✦

An improved method for the fractionation of human blood cells by centrifugal elutriation

✍ Scribed by W.L. van Es; W.S. Bont

Publisher: Elsevier Science
Year: 1980
Tongue: English
Weight: 375 KB
Volume: 103
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(80)90613-2

No coin nor oath required. For personal study only.

✦ Synopsis

Minor alterations of the electrical circuitry of the centrifuge required for centrifugal elutriation are described. Furthermore it is shown that elutriation can be performed by hydrostatic pressure without the utilization of a pump. This approach enables us to fractionate human blood cells at a constant flow rate by changing the rotor speed.

📜 SIMILAR VOLUMES

Centrifugal elutriation as a method for

Centrifugal elutriation as a method for isolation of large numbers of functionally intact human peripheral blood monocytes

✍ Jim Turpin; Jeane P. Hester; Evan M. Hersh; Gabriel Lopez-Berestein 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 656 KB

Centrifugal elutriation was used further to isolate human peripheral blood monocytes (HPBM) from mononuclear-enriched cells harvested as a secondary component following platelet concentration collection samples. HPBM were recovered in either one or two populations consisting of either total HPBM or

Stimulatory effect of counterflow centri

Stimulatory effect of counterflow centrifugal elutriation in large-scale separation of peripheral blood monocytes can be reversed by storing the cells at 37°c

✍ Tore G. Abrahamsen; Charles S. Carter; Elizabeth J. Read; Marc Rubin; Holly G. G 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 568 KB

Transfusion of peripheral blood monocytes may be of benefit as adjuvant treatment of leukopcnic patients with scrious infections. To study the feasibility of this approach, a largc-scale monocyte separation procedure employing leukapheresis. density gradient centrifugation. and counterflow centrifug

Dissection of the human brain as a metho

Dissection of the human brain as a method for its fractionation by weight

✍ Jenkins, Thomas W. ;Truex, Raymond C. 📂 Article 📅 1963 🏛 John Wiley and Sons 🌐 English ⚖ 568 KB

An improved method for purification of t

An improved method for purification of the cold-insoluble globulin of human plasma (Clg)

✍ Anthony B. Chen; Michael W. Mosesson 📂 Article 📅 1977 🏛 Elsevier Science 🌐 English ⚖ 548 KB

of the cold-insoluble globulin of human plasma (CIg) are described which result in a much higher recovery than formerly obtained.

An improved protocol for the preparation

An improved protocol for the preparation of yeast cells for transformation by electroporation

✍ Thompson, J. R.; Register, E.; Curotto, J.; Kurtz, M.; Kelly, R. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 98 KB 👁 2 views

Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f

An improved method for determining the a

An improved method for determining the actin filament content of nonmuscle cells by the DNase I inhibition assay

✍ Joan E.B. Fox; Michael E. Dockter; David R. Phillips 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 624 KB

The actin filament content of cell lysates can be assayed by inhibition of DNase I activity (Blikstad, I., Markey, F., Carlsson, L., Persson, T., and Lindberg, U. (1978) Cell 15, 935-943). We have modified this assay in two ways. We have (i) established lysis conditions that do not affect the state