✦ LIBER ✦
An improved method for determining the actin filament content of nonmuscle cells by the DNase I inhibition assay
✍ Scribed by Joan E.B. Fox; Michael E. Dockter; David R. Phillips
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 624 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
The actin filament content of cell lysates can be assayed by inhibition of DNase I activity (Blikstad, I., Markey, F., Carlsson, L., Persson, T., and Lindberg, U. (1978) Cell 15, 935-943). We have modified this assay in two ways. We have (i) established lysis conditions that do not affect the state of polymerization of actin and (ii) used a computer for direct data acquisition and analysis of the linear portion of the reaction curve. These modifications allow the accurate determination of small changes in the actin filament content of intact cells.