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Resolution of ampholytes focused in a natural pH gradient moving in a tapered capillary

✍ Scribed by K. Šlais

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
684 KB
Volume
7
Category
Article
ISSN
1040-7685

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✦ Synopsis

Abstract

Changes in the resolution of peaks of ampholytes during their migration and focusing in a natural pH gradient which moves electrophoretically in a tapered capillary are discussed. The influence of the capillary geometry on the needed potential drop as well as on the pressure drop accompanied by the renewing of the electrolytes is estimated. The expected performance of focusing according to the suggested model is related to that of focusing of the ampholytes in a pH gradient moving in a capillary of constant cross‐section.

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