Electrophoretic focusing in a natural steady state moving pH gradient

✍ Scribed by Karel Šlais

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: English
• Weight: 761 KB
• Volume: 5
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220050511

✦ Synopsis

Abstract

The physical and mathematical basic of a natural steady state, moving pH gradient is outlined. With certain approximations, a differential equation of the concentration of the component contributing to the natural steady state, moving pH gradient is formulated. Solutions of the differential equation and the relationships to the resolution calculations of both carriers and trace components present in the large excesses of carriers are presented. The steady state distribution profiles of both amphoteric and nonamphoteric components predict Gaussian distributions only if and when the conductivity and the pH gradient remain constant within the moving focused zone. The practical significance of the model is shown by its application to isoelectric focusing (IEF) with electrophoretic mobilization, to isotachophoresis (ITP) with polyampholytic spacers, and to related methods. It is shown that the presented approximate model represents the transition between classical models of IEF and ITP.