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Resistance to the anti-proliferative effect of IL-1 on human melanoma cell line is associated with endogenous production of IL-1 and IL-6

โœ Scribed by Makoto Araki; Taishi Yano; Hidetoshi Hayashi; Takemasa Takii; Katsuya Suzuki; Kikuo Onozaki

Publisher
John Wiley and Sons
Year
1994
Tongue
French
Weight
968 KB
Volume
56
Category
Article
ISSN
0020-7136

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โœฆ Synopsis

A human melanoma cell line (A375-6) became resistant to the anti-proliferative effect of human IL-l after a long period of culture. Two stable resistant sub-clones were obtained, and the mechanism of the IL-I resistance was investigated. Resistant cells, but not sensitive cells, appeared to produce constitutively IL-I activity. The activity was neutralized by anti-IL-I cu antibody but not by anti-IL-If3 antibody. Resistant cells expressed IL-I cu but not IL-I p mRNA. Therefore, the resistant cells appeared to produce IL-I (y. mRNA for IL-I receptor antagonist (IL-I Ra) was not detected in resistant cells, indicating that the resistance is not attributable to IL-IRa. These resistant cells were also resistant to the anti-proliferative effect of human IL-6, but not to that of human TNF. Resistant cells appeared to produce constitutively IL-6 more than sensitive cells, and IL-6 production both in sensitive and in resistant cells was augmented by exogenous IL-I. Furthermore, constitutive production of IL-6 in resistant cells was inhibited by IL-IRa. Type I IL-l receptor (IL-I R) mRNA was expressed equally in resistant and sensitive cells. These data indicate that the resistance is not the result of loss of functional IL-I R and that IL-I induces IL-6 in an autocrine manner. It is, therefore, conceivable that endogenous IL-I and IL-6 contribute to IL-I resistance.

๐Ÿ“œ SIMILAR VOLUMES

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## FOR ACQUIRED RESISTANCE OF HUMAN A375 MELANOMA The proliferation of human melanoma cell line A375-6 is inhibited by interleukin I (IL-I). However, the cells acquired resistance to IL-I after a long period of culture. We have reported that 2 resistant subclones, A375-R8 and -R19, produced ILla c

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