The effect of coculture with human smooth muscle cells on the proliferation, the IL-1 β secretion, the PDGF production and tube formation of human aortic endothelial cells

✍ Scribed by Hideki Asakawa; Tetsuro Kobayashi

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 744 KB
• Volume: 17
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/(sici)1099-0844(199906)17:2<123::aid-cbf817>3.0.co;2-3

✦ Synopsis

Endothelial cells (ECs) and smooth muscle cells (SMCs), which are the major component cells of blood vessels, produce various bioactive substances and communicate with each other through them. Although several studies of the interaction between ECs and SMCs have been reported, the eect of coculture with SMCs on ECs is still obscure. To clarify the interaction of ECs and SMCs, we examined the eect of coculture with SMCs on the proliferation, the IL-1 b secretion, the PDGF production and tube formation of ECs, using the coculture model: transferable wells and collagen gel. IL-1 and PDGF are considered to be related to progression of atherosclerosis. Proliferation and tube formation of ECs are associated with repair of vessels. In the transferable well system coculture with SMCs stimulated the proliferation of ECs, and enhanced the IL-1 b secretion of ECs and in the collagen gel system coculture with SMCs induced the tube formation of ECs, and appeared to enhance the PDGF production of ECs. In conclusion, the eect of coculture with SMCs on ECs has two con¯icting aspects: progression of atherosclerosis and angiogenesis. These results suggest that an imbalance of their eects may lead to pathological events.