Cell-free synthesis of preparative amounts of interleukin-6 (IL-6) in a wheat germ extract is described. Following transcription of uncapped mRNA by SP6 polymerase, protein was synthesized in a continuous flow of translation components. The system remained active for at least \(50 \mathrm{~h}\), pro
β¦ LIBER β¦
Requirement of Continuous Transcription for the Synthesis of Sufficient Amounts of Protein by a Cell-Free Rapid Translation System
β Scribed by Mami Hino; Yasuo Shinohara; Kazuaki Kajimoto; Hiroshi Terada; Yoshinobu Baba
- Book ID
- 115645973
- Publisher
- Elsevier Science
- Year
- 2002
- Tongue
- English
- Weight
- 77 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1046-5928
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