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A simple regression method for determining the quantity of translated protein in a cell-free protein synthesis system

✍ Scribed by Taek Jin Kang; Sang Hyeon Kang; Sang Taek Jung; Eun Yeol Lee; Cha Yong Choi


Book ID
114398642
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
362 KB
Volume
88
Category
Article
ISSN
1389-1723

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A cell-free translation system producing mature green fluorescent protein (GFP) can be a useful tool for studying the mechanism and kinetics of GFP chromophore formation, as well as for fast protein engineering. We report here that the mature GFP can be formed in the cellfree translation system from