Repression and activation of arginine transport genes in Escherichia coli K 12 by the ArgP protein
โ Scribed by Roberto T.F Celis
- Book ID
- 115629825
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 238 KB
- Volume
- 294
- Category
- Article
- ISSN
- 0022-2836
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Phi80dargECBH DNA has been used to direct cell-free synthesis of argininosuccinase, the argH gene product in Escherichia coli K12. In vitro enzyme synthesis is sensitive to repression by partially purified preparations from an argR+ strain but not by corresponding preparations from an argR- strain.
The regulation of synthesis of arg enzymes in Salmonella typhimurium by the arginine repressor of Escherichia coli K-12 has been reevaluated using a strain of S. typhimurium in which the argR gene was rendered nonfunctional by inserting the translocatable tetracycline-resistance element Tn10 into th