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In vitro synthesis and repression of argininosuccinase in Escherichia coli K12; partial purification of the arginine repressor

✍ Scribed by Kelker, Norman E. ;Maas, Werner K. ;Yang, Huey-Lang ;Zubay, Geoffrey

Publisher
Springer
Year
1976
Tongue
English
Weight
372 KB
Volume
144
Category
Article
ISSN
0026-8925

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✦ Synopsis

Phi80dargECBH DNA has been used to direct cell-free synthesis of argininosuccinase, the argH gene product in Escherichia coli K12. In vitro enzyme synthesis is sensitive to repression by partially purified preparations from an argR+ strain but not by corresponding preparations from an argR- strain. Using DNA-cellulose chromatography, approximately seventyfold purification of repressor has been obtained. The partially purified preparation represses argininosuccinase synthesis but has no effect on beta-galactosidase synthesis.

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