Two factor transductional crosses place recF at approximately 82 min on the E. coli chromosome; recF is highly cotransducible with dnaA and gyrB (cou). Transductional analysis with a series of lambda tna specialized transducing phages carrying chromosomal DNA from the tnaA region place recF between
Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12
✍ Scribed by Mergeay, Max ;Gigot, Daniel ;Beckmann, Jacques ;Glansdorff, Nicolas ;Piérard, André
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 942 KB
- Volume
- 133
- Category
- Article
- ISSN
- 0026-8925
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Using a modified lambda phage as a vector and a procedure developed in Dr. C. Schnaitman's laboratory, we have cloned the structural gene for protein K from an Escherichia coli K1 strain to an E coli K-12 strain. The cloned inserts consist of two HindIII fragments, 4 kb and 6.5 kb in size. The prote