𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Genetic and physical mapping of recF in Escherichia coli K-12

✍ Scribed by Ream, Lloyd Walter ;Margossian, Linda ;Clark, Alvin J. ;Hansen, Flemming G. ;von Meyenburg, Kaspar

Publisher
Springer
Year
1980
Tongue
English
Weight
866 KB
Volume
180
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

✦ Synopsis

Two factor transductional crosses place recF at approximately 82 min on the E. coli chromosome; recF is highly cotransducible with dnaA and gyrB (cou). Transductional analysis with a series of lambda tna specialized transducing phages carrying chromosomal DNA from the tnaA region place recF between dnaA and gyrB. This analysis also indicates that a gene lying in the same region and producing an easily detectable protein (estimated MW of 45 kD) is dnaN and not recF.

πŸ“œ SIMILAR VOLUMES

Physical mapping of the gene for aminope
Physical mapping of the gene for aminopeptidase N in Escherichia coli K12
✍ Bally, M. ;Murgier, M. ;Tommassen, J. ;Lazdunski, A. πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 139 KB

The pepN gene has been cloned into the multicopy plasmid pBR322. The restriction map of the insert was established and the gene was localized. By comparison with the restriction map of the plasmid pJP30 bearing the ompF region, it has been possible to order the ompF, asnS, and pepN genes. The ompF a

Genetic mapping of a putative temperatur
Genetic mapping of a putative temperature-sensitive transcription mutation in Escherichia coli K12
✍ Abdul Jabbar, M. ;Jayaraman, R. πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 531 KB

A putative temperature-sensitive transcription mutant described earlier (Jabbar and Jayaraman, 1976) has been genetically mapped. The locus maps at 38 min to the left of aroD. The mutation is recessive to the wild type and it affects a gene probably other than the genes coding for the alpha and beta