Reply to the letter by MacLeod et al.

✍ Scribed by Hisashi Kubota; Takafumi Nishizaki; Kohsuke Sasaki

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 28 KB
• Volume: 33
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.1218

✦ Synopsis

We are grateful to Dr. MacLeod and his colleagues for raising this important suggestion. As they commented, in several cases it is not possible to rule out accidental inclusion of syngeneic subclones in the analysis due to cell line cross-contamination. Therefore, such cell line cross-contamination would appear to have affected the accuracy of the estimated frequency of recurrent derivative chromosomes in our experiment. However, most of the examined cell lines revealed quite different chromosomal rearrangements. Even if cell lines having similar alterations detected by SKY were excluded from these analyses, the present results still reveal a tendency for a close relationship between DNA amplification and chromosomal translocation in glioblastoma.