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Reply to the letter by Dr. Palmiere and Dr. Mangin

✍ Scribed by Takahito Hayashi; Kazutoshi Ago; Mihoko Ago; Mamoru Ogata

Book ID: 113773400
Publisher: Elsevier Science
Year: 2012
Tongue: English
Weight: 128 KB
Volume: 14
Category: Article
ISSN: 1344-6223
DOI: 10.1016/j.legalmed.2012.04.001

No coin nor oath required. For personal study only.

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Dr. Vinogradov points out that propidium iodide (PI) binding data do not always reflect accurate genome size values. PI binding can be influenced by the chromatin conformation. In addition, PI is not DNA specific and pretreatment of samples with RNase can affect the fluorescence intensity in an unpr