Replication of Human Syncytium-Forming Virus in Human Cells: Effect of Certain Biological Factors and Selective Chemicals

✍ Scribed by Dr. Philip C. Loh; Kheng See Ang

• Publisher: John Wiley and Sons
• Year: 1981
• Tongue: English
• Weight: 438 KB
• Volume: 7
• Category: Article
• ISSN: 0146-6615
• DOI: 10.1002/jmv.1890070108

✦ Synopsis

Abstract

The growth characteristics of the human syncytium‐forming virus (HSFV) were examined in several human cell lines of normal and malignant origins and composing of either fibroblastic or epithelial‐like cells. Virus production occurred only in the fibroblastic diploid cell lines: HEF (human embryonic cells, Flow #5,000) and HFDL #645 (human fetal diploid lung), but not in the epithelial‐like heteroploid cell lines: RA (a continuous line of human amnion), #999 (human bone marrow), and KB (carcinoma of the nasopharynx). While the single‐cycle growth pattern of the virus in HEF and HFDL #645 cell lines were essentially similar, the virus yield per cell was greater in the HFDL #645 cells. Furthermore, the physiological state of the cell had a marked effect on virus production. Subconfluent actively growing HFDL #645 cells produced higher yields of virus than density‐inhibited confluent HFDL #645 cell cultures. The replication of HSFV was inhibited by actinomycin D at concentrations that did not interfere with poliovirus replication (0.001 to 0.01 jug/ml). Pretreatment and posttreatments of infected cell cultures with either the polycation polybrene (hexadimethrine bromide) or the synthetic glucocorticosteroid dexamethasone did not enhance HSFV production.