Comparative inhibitory effects of suramin and other selected compounds on the infectivity and replication of human T-cell lymphotropic virus (HTLV-III)/lymphadenopathy-associated virus (LAV)
✍ Scribed by Jan Balzarini; Hiroaki Mitsuya; Erik De Clercq; Samuel Broder
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- French
- Weight
- 681 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
Suramin and various other selected compounds were evaluated for their in vitro inhibitory effects on the infectivity and replication of human T-cell lymphotropic virus (HTLVIlll)/lymphadenopathy-associated virus (LAV). As parameters for infectivity and replication, respectively, we followed the cytopathic effect of HTLV-IIIILAV on ATH 8 cells, a T-cell clone with high susceptibility to HTLV-IIIILAV, and the expression of HTLV-IIIILAV p24 gag protein in H9 cells infected with HTLV-IIIILAV. As the most effective inhibitors of HTLV-IIIILAV the following substances emerged (in order of decreasing activity): Evans Blue = suramin > phosphonoforrnic acid > Direct Yellow 50. Several purine nucleoside analogues including vidarabine, tubercidin, neplanocin A, dihydroxypropyladenine, pyrazofurin and ribavirin were not inhibitory to HTLV-IIIILAV. In our test systems, involving a high multiplicity of infection, HPA-23, previously reported to be effective against LAV reverse transcriptase, showed no inhibitory effect on HTLV-IIIILAV infectivity for ATH 8 cells and proved only weakly inhibitory to HTLV-IIIILAV replication in H9 cells. Thus, among the anionic dyes that are structurally related to suramin, compounds were found which were as active as surarnin itself, if not more so.