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Replication kinetics of X chromosomes in fibroblasts and lymphocytes

โœ Scribed by K. S. Reddy; J. R. K. Savage; D. G. Papworth

Publisher
Springer
Year
1988
Tongue
English
Weight
488 KB
Volume
79
Category
Article
ISSN
0340-6717

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โœฆ Synopsis

The kinetics of replication for early and late replicating X chromosomes in karyotypically normal fibroblasts and lymphocytes was studied using terminal bromodeoxyuridine (BrdU) treatment followed by Hoechst/light/Giemsa staining. Although the order of band appearance differs between the two tissues, the programme (order and interval between band appearances) for early replicating bands (dark R-bands) is identical in the two homologues. This is probably also the case for later replicating bands (dark G-bands) though the criteria for determining mean band appearance times are less reliable for these bands when terminal BrdU treatment is used. This means that the late X has a delayed start but thereafter proceeds at the same pace as its early counterpart.

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