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Bloom's syndrome: DNA replication in cultured fibroblasts and lymphocytes

✍ Scribed by Roger Hand; James German

Publisher: Springer
Year: 1977
Tongue: English
Weight: 639 KB
Volume: 38
Category: Article
ISSN: 0340-6717
DOI: 10.1007/bf00402156

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✦ Synopsis

Analysis of DNA fiber autoradiograms from Bloom's syndrome skin fibroblasts and blood lymphocytes shows a retarded rate of replication fork movement compared to normal adult controls. Other measurements from the autoradiograms--replication unit length, incidence of bidirectional replication, and degree of initiation synchrony--are normal in Bloom's syndrome cells. These results suggest that a slow rate of fork movement is a specific manifestation of defective DNA synthesis in all Bloom's syndrome cells.

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