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Repetitive use of polyacrylamide gels for the analysis and purification of oligonucleotides

✍ Scribed by Gaytán, Paul; López-Bustos, Eugenio; Güereca, Leopoldo

Book ID
125424118
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
592 KB
Volume
439
Category
Article
ISSN
0003-2697

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A nondenaturing gradient polyacrylamide gel electrophoresis method is described for the resolution of membrane proteins. Bovine heart inner mitochondrial membranes were solubilized in Triton X-100 and individual complexes were identified by staining for activity and protein. Succinate dehydrogenase