Location and purification of enzymes on polyacrylamide gels using dialyzable fluorescent markers
โ Scribed by A.M. Bodhe; H.G. Vartak; M.V. Rele; V.S. Dhamankar
- Book ID
- 102988611
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 753 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The characteristics of protein detection and quantitation with SYPRO Ruby protein gel stain in one- and two-dimensional polyacrylamide gels were evaluated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of three different purified recombinant proteins showed that the l
We describe a method for the detection of isoforms of several glycolytic enzymes by activity staining after native PAGE. The staining is based on coupled enzyme assays carried out on the gel after electrophoresis and is linked to the disappearance of NADH, which is visualized by fluorescence. This m