Removal of the 33, 23 and 18 kDa extrinsic proteins of photosystem II by sulfite treatment at alkaline pH
โ Scribed by M. Beauregard; L. Morin; R. Popovic
- Book ID
- 117057809
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 307 KB
- Volume
- 152
- Category
- Article
- ISSN
- 0006-291X
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This communication introduces a new spectrophotometric assay for the detection of peroxide generated by Photosystem II (PS II) under steady state illumination in the presence of an electron acceptor. The assay is based on the formation of an indamine dye in a horseradish peroxidase coupled reaction
A highly purified oxygen evolving Photosystem II core complex was isolated from PS II membranes solubilized with the non-ionic detergent n-octyl-/~-D-thioglucoside. The three extrinsic proteins (33, 23 and 17 kDa) were functionally bound to the PS II core complex. Selective extraction of the 22, 10