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Selective extraction of CP 26 and CP 29 proteins without affecting the binding of the extrinsic proteins (33, 23 and 17 kDa) and the DCMU sensitivity of a Photosystem II core complex

✍ Scribed by Ranjit K. Mishra; Demetrios F. Ghanotakis

Publisher: Springer
Year: 1994
Tongue: English
Weight: 811 KB
Volume: 42
Category: Article
ISSN: 0166-8595
DOI: 10.1007/bf00019056

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✦ Synopsis

A highly purified oxygen evolving Photosystem II core complex was isolated from PS II membranes solubilized with the non-ionic detergent n-octyl-/~-D-thioglucoside. The three extrinsic proteins (33, 23 and 17 kDa) were functionally bound to the PS II core complex. Selective extraction of the 22, 10 kDa, CP 26 and CP 29 proteins demonstrated that these species are not involved in the binding of the extrinsic proteins (33, 23 and 17 kDa) or the DCMU sensitivity of the Photosystem II complex.

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