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Removal of specific protein spots on the patterns of non-denaturing two-dimensional electrophoresis using protein A agarose and antibodies

✍ Scribed by Youji Shimazaki; Seiichi Ohara; Takashi Manabe


Book ID
117331494
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
157 KB
Volume
37
Category
Article
ISSN
0165-022X

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A new method for visualizing 2D protein spot patterns is described whereby proteins containing sulfhydryl groups are labeled with the fluorophore monobromobimane prior to the isoelectric focusing step of 2D polyacrylamide gel electrophoresis. The method requires the addition of a single reagent and