Comparative fluorescence two-dimensional gel electrophoresis using a gel strip sandwich assembly for the simultaneous on-gel generation of a reference protein spot grid

Two-Dimensional Polyacrylamide Gel Elect

Two-Dimensional Polyacrylamide Gel Electrophoresis of Proteins Labeled with the Fluorophore Monobromobimane Prior to First-Dimensional Isoelectric Focusing: Imaging of the Fluorescent Protein Spot Patterns Using a Cooled Charge-Coupled Device

✍ V.E. Urwin; P. Jackson 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 765 KB

A new method for visualizing 2D protein spot patterns is described whereby proteins containing sulfhydryl groups are labeled with the fluorophore monobromobimane prior to the isoelectric focusing step of 2D polyacrylamide gel electrophoresis. The method requires the addition of a single reagent and