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Removal of RNA from DNA preparations by the addition of lysine-rich histone

✍ Scribed by M. Sluyser; L. Den Engelse

Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
186 KB
Volume
25
Category
Article
ISSN
0003-2697

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✦ Synopsis

Most procedures for the isolation of DNA have been designed for the extraction of this material from calf thymus glands. Difficulties are, however, encountered when similar procedures are applied to isolate DNA from tissues such as rat and mouse liver, since the DNA is usually contaminated with RNA, part of which is resistant ta digestion with ribonuclease. Butler and Johns (1) reported that calf thymus DNA could be separated from added low molecular weight rat liver RN-4 by precipitation with lysine-rich calf thymus histone. We have found that this method can be used to purify preparations of liver DNA, since ribonuclease-resistant RNA is also detached from DNA. This appears to be a rapid and mild procedure which is especially useful when one wants to compare the i'n viva labeling of DNA and RNA.

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