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Removal of detergents from protein extracts using activated charcoal prior to immunological analysis

✍ Scribed by Ashraf N. Malhas; Ramadan A. Abuknesha; Robert G. Price

Book ID
114051025
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
182 KB
Volume
264
Category
Article
ISSN
0022-1759

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The method for extracting Triton X-100 used by I. H. Mather and C. B. Tampling [Anal. B&hem. 93, 139-142 (1979)], has been extended to other detergents of dif%erent charge and chemical nature. All the detergents tested can be extracted with isopentanol in conditions in which not more than 8% of hydr