Extraction of detergents from hydrophobic proteins with isopentanol: Application to electrophoretic analysis of photosynthetic bacterial hydrophobic proteins
✍ Scribed by Jorge Ramírez Salcedo; Rubén Salcedo Hernández; Heliodoro Célis
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 291 KB
- Volume
- 132
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The method for extracting Triton X-100 used by I. H. Mather and C. B. Tampling [Anal. B&hem. 93, 139-142 (1979)], has been extended to other detergents of dif%erent charge and chemical nature. All the detergents tested can be extracted with isopentanol in conditions in which not more than 8% of hydrophobic or hydrophilic protein is lost from the water phase. The removal of detergent from reaction centers and light harvesting protein-pigment complexes of photosynthetic bacteria, eliminates the artifacts of oligomers when analyzed by sodium dodecyl sulfate-gel electrophoresis.
KEY WORDS detergent extraction; isopentanol; hydrophobic proteins; elcctrophorcsis; photosynthetic; bacteria.