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Reliable determination of transposon insertion site in prokaryotes by direct sequencing

โœ Scribed by Udi Qimron; Neta Madar; Rivi Ascarelli-Goell; Maya Elgrably-Weiss; Shoshy Altuvia; Angel Porgador


Book ID
117360122
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
174 KB
Volume
54
Category
Article
ISSN
0167-7012

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Tagged transposons are powerful tools for large-scale studies of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. The current techniques used to identify transposon insertion sites in the yeast genome require a DNA ampliยฎcation step that can be time-consuming a