Identifying tagged transposon insertion sites in yeast by direct genomic sequencing
✍ Scribed by Joe Horecka; Yoshifumi Jigami
- Book ID
- 101322388
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 100 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0749-503X
No coin nor oath required. For personal study only.
✦ Synopsis
Tagged transposons are powerful tools for large-scale studies of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. The current techniques used to identify transposon insertion sites in the yeast genome require a DNA ampli®cation step that can be time-consuming and problematic. We show that the DNA ampli®cation step can be bypassed. Insertion sites can be identi®ed rapidly and reliably by direct genomic sequencing using a transposon-speci®c primer, BigDye-labelled terminators, and an automated sequencer. Direct genomic sequencing can also save time on the genetic analysis phase of transposon-based projects.