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“Release radioimmunoassay”: Description of a new method and its application to the determination of human chorionic somatomammotropin plasma levels

✍ Scribed by F. Cocola; A. Orlandini; G. Barbarulli; P. Tarli; P. Neri

Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
553 KB
Volume
99
Category
Article
ISSN
0003-2697

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✦ Synopsis

A new radioimmunoassay (RIA) method is proposed, utilizing cellulose disks coated with a '%Ag/Ab complex as a single reagent. The labeled antigen is released into the incubation medium proportionally to the concentration of the corresponding antigen in the sample. The method is comparable to the classical RIA with regard to sensitivity, precision, and specificity, but it has the advantage of a wide range of nondependence on the sample volume and greater practicability (the analysis is performed using a single-step procedure). The assay of human chorionic somatomammotropin is adopted as a model.

'251-Ag/Ab AglAb I (insolubilized) + Ag + 1251-Ag.

Methods

Hormones. HCS and HGH were prepared and characterized in our laboratories (11-14).

Labeled hormone. 1251-HCS was prepared using the enzymatic labeling technique

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