The kinetics of renaturation of heat-or formamidedenatured DNA have been studied by following the change of optical density at a constant temperature. Solvents of different ionic strength and various DNA samples have been used. At the lower ionic strength studied, the reaction follows second-order k
Relaxation kinetics of denaturation of DNA
β Scribed by Narendra S. Goel
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1968
- Tongue
- English
- Weight
- 848 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0006-3525
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β¦ Synopsis
The one-dimensional Ising model, with nearest-neighbor correlations only, used earlier in equilibrium studies of melting of DNA is extended to study the relaxation kinetics of copolymeric synthetic DXA near the melting temperature. An exponential kinetics, in agreement with the observations, has been found.
π SIMILAR VOLUMES
In a continuing study of the denaturation of DNA as brought about by Cu(I1) ions, results are presented for the dependence of T , and T (the terminal relaxation time) on ionic strength, pH, reactant concentrations, and temperature. Maximum stability of the double helix, as reflected by the longest r
The structure of renatured T4 DNA has been studied by CsCl density-gradient centrifugation. It has, been found that the products of the reaction differ, depending on the method used for denaturation of the DNA. If denaturation is carried out without taking precautions to prevent chain degradation, f
## Abstract The influence of singleβstrand breaks on the kinetics of the relaxation of DNA in a solution of low ionic strength has been investigated by a temperature jump method. The relaxation of DNA after a jump of 0.7 Β°C in the melting region has been monitored by measuring the extinction at 260