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Copper(II)—DNA denaturation. II. The model of DNA denaturation

✍ Scribed by Donald C. Liebe; John E. Stuehr

Publisher
Wiley (John Wiley & Sons)
Year
1972
Tongue
English
Weight
981 KB
Volume
11
Category
Article
ISSN
0006-3525

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✦ Synopsis

In a continuing study of the denaturation of DNA as brought about by Cu(I1) ions, results are presented for the dependence of T , and T (the terminal relaxation time) on ionic strength, pH, reactant concentrations, and temperature. Maximum stability of the double helix, as reflected by the longest relaxation times and highest T, values, was observed between pH 5.3 and 6.2. Outside this range both T, and T decreased sharply.

A second, faster relaxation time was deduced from the kinetic curves. The apparent activation energies of the rapid and slow ("terminal") relaxations were found to be 12 and 55 kcal/mole, respectively. Several lines of evidence led to the conclusions that (1) the rate-determining step in DNA denaturation, when occurring in the transition region, is determined by chemical events and ( 2 ) the interactions which are disrupted kinetically in the rate-determining step are those which account for the major portion of the thermal (T,) stability of helical DNA.

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