Relative efficacy of chelating agents on excretion and tissue distribution of manganese in mice

✍ Scribed by D. J. Sánchez; M. Gómez; J. L. Domingo; J. M. Llobet; J. Corbella

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 456 KB
• Volume: 15
• Category: Article
• ISSN: 0260-437X
• DOI: 10.1002/jat.2550150409

✦ Synopsis

The effect of repeated parenteral administration of a number of structurally diverse chelating agents on the excretion and tissue distribution of manganese was assessed in mice following 4 weeks of manganese exposure. Males Swiss mice received S.C. injections of manganese(I1) chloride tetrahydrate (8.9 mg Mn kg-' body wt.) for 4 weeks (5 days per week). After the end of this exposure period, cyclohexanediaminetetraacetic acid (CDTA), ethyleneglycol-bis-(B-aminoethylether)-~,~-tetraaceticacid (EGTA), K(2-hydroxyethyl)ethylenediamine triacetic acid (HEDTA), isonicotinyl hydrazine (INH), L-dopa, sodium 4,5-dihydroxy-l,3-benzenedisulphonate (Tiron), paminosalicylic acid (PAS) or 0.9% saline (control group) were given i.p. for five consecutive days. The doses of the chelators were approximately equal to one-eighth of their respective LD-values. Urine and faeces were daily collected for 5 days. Twenty-four hours after the final chelator injection, mice were killed and manganese concentrations were determined in various tissues. Although CDTA, EGTA and HEDTA significantly enhanced the elimination of manganese into urine, none of the chelators increased faecal excretion. Tissue concentrations of manganese were significantly reduced only by CDTA. According to these results, among the compounds tested only CDTA would mobilize effectively manganese in manganese-loaded mice. ~ ~ ~ ~~ EXPERIMENTAL Animals and chemicals Male Swiss mice (Interfauna Iberica, Barcelona, Spain) weighing 28-32 g were used. The animals were acclimatized to the conditions of the animal room (21-23"C, CCC 02W37W95/040285-04 0 1995 by John Wiley & Sons, Ltd.