In vivo Characterization of Gd(BME-DTTA), a myocardial MRI contrast agent: tissue distribution of its MRI intensity enhancement, and its effect on heart function
✍ Scribed by Wen-Jang Chu; Tamas Simor; Gabriel A. Elgavish
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 466 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0952-3480
No coin nor oath required. For personal study only.
✦ Synopsis
We have determined an LD 50 of 0.56 ± 0.05 mmol/kg for liposomal Gd(BME-DTTA) in mice and also shown that liposomal Gd(BME-DTTA) has no deleterious effects on heart rate, blood pressure, left ventricular force and AV conductance in ferret hearts in vivo at the magnetic resonance imaging (MRI)-effective dose of 0.05 mmol/kg body weight. In MRI images, a 1 H signal intensity enhancement is observed in the following organs in decreasing order of the effect: heart Ϸ spleen > kidney > liver. This enhancement is stable for over 3 h in all organs. The results of 1 H MRI and electron micrographs indicate that the lipophilic fatty acyl groups in the ligand BME structure and the particle sizes of liposomal Gd(BME-DTTA) are two important factors for tissue specificity of liposomal Gd(BME-DTTA) in the intensity enhancement. In vitro relaxivity of a liposomal Gd(BME-DTTA) sample, stored at 4°C, remained stable for over 4 months of observation, but a significant decrease in relaxivity was observed in a sample stored at room temperature, most likely reflecting some deterioration in liposome chemistry.