Relationship between the morphological changes of somatic compartment and the kinetics of nuclear and cytoplasmic maturation of oocytes during in vitro maturation of porcine follicular oocytes
✍ Scribed by Tamás Somfai; Kazuhiro Kikuchi; Akira Onishi; Masaki Iwamoto; Dai-ichiro Fuchimoto; Ágnes Bali Papp; Eimei Sato; Takashi Nagai
- Book ID
- 111663682
- Publisher
- John Wiley and Sons
- Year
- 2004
- Tongue
- English
- Weight
- 185 KB
- Volume
- 68
- Category
- Article
- ISSN
- 1040-452X
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Cumulus-enclosed sheep oocytes were cultured in gonadotrophin-containing medium for up to 9 hr and were then incubated for 3 hr in the presence of [32P]phosphate. The incorporation of 32P into TCA-insoluble material was measured, and oocyte proteins were separated by one-and twodimensional gel elect
## Abstract Meiotic maturation of mammalian oocytes is a complex process during which microfilaments and microtubules provide the framework for chromosomal reorganisation and cell division. The aim of this study was to use fluorescence and confocal laser scanning microscopy to examine changes in th