Changes in histone modifications during in vitro maturation of porcine oocytes
โ Scribed by Tsutomu Endo; Kunihiko Naito; Fugaku Aoki; Sachi Kume; Hideaki Tojo
- Book ID
- 111663842
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 176 KB
- Volume
- 71
- Category
- Article
- ISSN
- 1040-452X
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Cumulus-enclosed sheep oocytes were cultured in gonadotrophin-containing medium for up to 9 hr and were then incubated for 3 hr in the presence of [32P]phosphate. The incorporation of 32P into TCA-insoluble material was measured, and oocyte proteins were separated by one-and twodimensional gel elect
## Abstract The regulation of meiotic events of goat oocytes from prophase I to metaphase II was studied by inhibiting protein synthesis at different times of the transition and by analyzing the changes in the protein synthesis pattern during maturation. Protein synthesis was required for germinal