Relation of brain regional physostigmine concentration to cholinesterase activity and acetylcholine and choline levels in rat
β Scribed by Marta Hallak; Ezio Giacobini
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 576 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0364-3190
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π SIMILAR VOLUMES
Regional choline (Ch) and acetylcholine (ACh) in rat brain were clearly determined by high-performance liquid chromatography with electrochemical detection. The method is based on that of Potter et al.: the hydrogen peroxide that is enzymatically produced from both compounds is measured and a succes
H-labeled physostigmine (50 pg. kg-') was administered intravenously to rats, and its concentration in brain tissue and spinal cord was assessed by quantitative autoradiography. Regional cerebral blood flow (rCBF) was measured with i~do-'~C-antipyrine autoradiography in control rats and in animals i