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Determination of choline and acetylcholine levels in rat brain regions by liquid chromatography with electrochemical detection

✍ Scribed by Yasushi Ikarashi; Tomiya Sasahara; Yuji Maruyama

Publisher: Elsevier Science
Year: 1985
Tongue: English
Weight: 1011 KB
Volume: 322
Category: Article
ISSN: 1873-3778
DOI: 10.1016/s0021-9673(01)97671-x

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✦ Synopsis

Regional choline (Ch) and acetylcholine (ACh) in rat brain were clearly determined by high-performance liquid chromatography with electrochemical detection. The method is based on that of Potter et al.: the hydrogen peroxide that is enzymatically produced from both compounds is measured and a successful improvement of the method, particularly for purification, is described. Recoveries were 96.1 +/- 1.4% for Ch and 95.6 +/- 2.2% for ACh and amounts as low as 10 pmol could be determined. Prior to measuring the compounds, a newly developed magnetic field microwave instrument (10 kW) was utilized for the rapid inactivation of brain enzymes. The levels of Ch and ACh in brain regions were compared with those reported elsewhere.

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