Reinvestigation of the chlorophyll distribution among the chlorophyll-proteins and chlorophyll-protein complexes ofHordeum vulgareL.

✍ Scribed by J. -P. Noben; R. Valcke; M. Poucke; H. Clijsters

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 412 KB
• Volume: 4
• Category: Article
• ISSN: 0166-8595
• DOI: 10.1007/bf00041808

✦ Synopsis

Solubilization of barley (Hordeum vulgare L.) thylakoid membranes with sodium dodecylsulphate plus sodium deoxycholate with or without Triton X-100 and subsequent fractionation in the polyacrylamide gel electrophoresis system described in this paper resulted: (i) in the resolution of the chlorophyll-proteins and chlorophyllprotein complexes commonly known as CPla, CP1, LHCW, LHCP ~ , CPa and LHCP3 ;

(2) in the highly increased stability of CP1 and CPla, as judged by their chlorophyll content, (3) at the expense of the free pigment concentration (4) which could be reduced to a negligible amount. Some 40% of the total chlorophyll contained in the mature higher plant thylakoid membrane is associated with CP1 and CPla and as already suggested before [ 19] no significant amount of free chlorophyll occurs in vivo.