Reinigung der D-Oxynitrilase aus Mandeln mit Hilfe der Affinitäts-Chromatographie

✍ Scribed by Erich Hochuli

• Book ID: 102252820
• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: German
• Weight: 306 KB
• Volume: 66
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.19830660210

✦ Synopsis

A Rapid Purification of D‐Oxynitrilase from Almond Meal by Affinity Chromatography

Oxynitrilase from almond meal is capable of catalyzing the stereospecific addition of cyanide to a variety of aldehydes. Thus, the enzyme is potentially useful in the synthesis of optically active cyanohydrins on a preparative scale [1]. As the currently available purification procedures for this enzyme [2] are rather tedious, we have elaborated a simple and rapid procedure based on affinity chromatography. An inhibitor for the enzyme, methyl p‐(3‐aminopropoxy)benzoate (4), has been synthesized and attached covalently to Sepharose 4B as a solid matrix (5). With this affinity gel it was possible to prepare the D‐oxynitrilase in a simple procedure with high yields.