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Steroidumwandelnde Enzyme aus Mikroorganismen II. Reinigung einer 3-Oxosteroid: (2,6-Dichlorphenolindophenol) Δ1-Oxidoreduktase aus Nocardia opaca mit Hilfe der Affinitätschromatographie

✍ Scribed by Dr. C. Hörhold; P. Atrat; V. Deppmeyer; A. Gabert; Ch. Flemming; K. Schubert


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
169 KB
Volume
16
Category
Article
ISSN
0233-111X

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✦ Synopsis


Steroidumwandelnde Enzyme aus Mikroorganismen

  1. Reinigung einer 3-Oxosteroid: (2,6-Dichlorphnnolindophenol) d1-Oxidoreduktase aus Nocardia opaca mit Hilfe der Affinitatschromatographie C. HORHOLD, P. ATRAT, V. DEPPMEYER, A. GABERT, CH. FLEMMING und K. SCHUBERT (Eingegangen am 31.1.1976

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The 4-en-3-oxosteroid-5a-reductase from Mycobacterium smegmatis mas bound biospecifically on the affinant containing an immobilized testosterone ligand. The enzyme obtained by elution with ethylene glycol and urea in a 32 fold purity has a S. A. of 8.73 x p ~i androstenedione min-1 mg-'. The coenzy