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Regulation of the nitrogen fixation genes inKlebsiella pneumoniae: Implications for genetic manipulation

✍ Scribed by R. A. Dixon; M. Buck; M. Drummond; T. Hawkes; H. Khan; S. MacFarlane; M. Merrick; J. R. Postgate


Publisher
Springer
Year
1986
Tongue
English
Weight
473 KB
Volume
90
Category
Article
ISSN
0032-079X

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The glnA, ntrB and ntrC genes of Klebsiella pneumoniae have been cloned, on a 12 kb HindIII fragment, into the plasmid pACYC184. In a coupled in vitro transcription/translation system the resultant plasmid, pGE100, directed synthesis of five polypeptides (molecular weights 73, 53, 51, 39, 36 kd) fro