Regulation of skeletal muscle satellite cell proliferation and differentiation by transforming growth factor-beta, insulin-like growth factor I, and fibroblast growth factor

✍ Scribed by Ronald E. Allen; Linda K. Boxhorn

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 536 KB
• Volume: 138
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041380213

✦ Synopsis

Skeletal muscle satellite cells were cultured from mature rats and were treated in vitro with various combinations of transforming growth factor (TGF)-beta, fibroblast growth factor (FGF), and insulin-like growth factor I (IGF-l)~ In serum-free defined medium the following observations were made: TCF-beta depressed proliferation and inhibited differentiation; FCF stimulated proliferation and depressed differentiation; IGF-I stimulated proliferation to a small dcgree but demonstrated a more pronounced stimulation of differentiation. In evaluating Combinations of these three factors, the differentiation inhibiting effect of TGF-beta could not be counteracted by any combination of IGF-l or FGF. The proliferation-depressing activity of TGF-beta, however, could not inhibit the mitogenic activity of FGF. Maximum stimulation of proliferation was observed in the presence of both FGF and IGF-I. The highest percentage fusion was also observed under these conditions, but differentiation with minimal proliferation resulted from treatment with IGF-I, alone. By altering the concentrations of TGF-beta, FGF, and IGF-I, satellite cells can be induced to proliferate, differentiate, or to remain quiescent.