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Regulation of polyamine transport by polyamines and polyamine analogs

✍ Scribed by D. L. Kramer; J. T. Miller; R. J. Bergeron; R. Khomutov; A. Khomutov; C. W. Porter

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
947 KB
Volume
155
Category
Article
ISSN
0021-9541

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✦ Synopsis

Regulation of polyamine transport in murine L1210 leukemia cells was characterized in order to better understand its relationship to specific intracellular polyarnines and their analogs and to quantitate the sensitivity by which it is controlled. Up-regulation of polyamine uptake was evaluated following a 48-hr treatment with a combination of biosynthetic enzyme inhibitors to deplete intracellular polyamine pools. The latter declined gradually over 48 hr and was accompanied by a steady increase in spermidine (SPD) and spermine (SPM) transport as indicated by rises in V , , , ,

to levels -4.5 times higher than control values. Restoration of individual polyamine pools during a 6-hr period following inhibitor treatment revealed that SPD and SPM uptake could not be selectively affected by specific pool changes. The effectiveness of individual polyamines in reversing inhibitorinduced stimulation of uptake was as follows: putrescine < SPD < SPM = the SPM analog, N',N'2-bis(ethyl)spermine (BESPM). In contrast to stimulation of transport, down-regulation by exogenous polyamines or analogs occurred rapidly and in response to subtle increases in intracellular pools. Following a 1 -hr exposure to 10 KM BESPM, V , , , values for SPD and SPM fell by 70%, whereas the

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